ARG10719

anti-MAP2ab antibody [4H5]

anti-MAP2ab antibody [4H5] for ICC/IF,IHC-Frozen sections,Western blot and Bovine,Human,Mouse,Rat

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody

Overview

Product Description Mouse Monoclonal antibody [4H5] recognizes MAP2ab
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, IHC-Fr, WB
Specificity This antibody was raised against purified full length bovine brain MAP2 and the epitope was mapped to aa. 631-1056 of the human MAP2 sequence, so it reacts to MAP2a and MAP2b proteins.
Host Mouse
Clonality Monoclonal
Clone 4H5
Isotype IgG1
Target Name MAP2ab
Antigen Species Human
Immunogen Full length purified Bovine protein.
Epitope Epitope mapped to projection domain of Human sequence, between aa. 631-1056.
Conjugation Un-conjugated
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17756 Mouse MAP2

GeneID: 25595 Rat MAP2

GeneID: 281294 Bovine MAP2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
Highlight Related products:
MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Calculated MW 200 kDa
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).