ARG11089

anti-MAP2ab antibody

anti-MAP2ab antibody for Western blot,IHC-Frozen sections and Mouse,Rat

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody

Overview

Product Description Goat Polyclonal antibody recognizes MAP2ab
Tested Reactivity Ms, Rat
Tested Application IHC-Fr, WB
Specificity The antibody detects MAP2a and MAP2b isoforms but not the MAP2c and MAP2d which lack projection domain sequences.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MAP2ab
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 377-1505 (projection domain sequence) of Human MAP2.
Conjugation Un-conjugated
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:1000 - 1:2000
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17756 Mouse MAP2

GeneID: 25595 Rat MAP2

Swiss-port # P15146 Rat Microtubule-associated protein 2

Swiss-port # P20357 Mouse Microtubule-associated protein 2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. Cell projection, dendrite. [UniProt]
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MAP2 antibodies; MAP2 Duos / Panels; Anti-Goat IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Calculated MW 200 kDa
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). [UniProt]