ARG63068

anti-MAP2ab antibody [MT-07]

anti-MAP2ab antibody [MT-07] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Pig

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody

Overview

Product Description

Mouse Monoclonal antibody [MT-07] recognizes MAP2ab

Tested Reactivity Hu, Ms, Pig
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The clone MT-07 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab).
Host Mouse
Clonality Monoclonal
Clone MT-07
Isotype IgG1
Target Name MAP2ab
Immunogen Microtubule protein (bovine brain) enriched for kinesin
Conjugation Un-conjugated
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB, IP and ELISA: Porcine brain
IHC-P: Brain
IHC-Fr: Mouse brain
ICC/IF: SH-SY5Y

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17756 Mouse MAP2

GeneID: 4133 Human MAP2

Swiss-port # P11137 Human Microtubule-associated protein 2

Swiss-port # P20357 Mouse Microtubule-associated protein 2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
Highlight Related products:
MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Calculated MW 200 kDa
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).