ARG43156
anti-MAP3K1 / MEKK1 antibody
anti-MAP3K1 / MEKK1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MAP3K1 / MEKK1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAP3K1 / MEKK1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1400 to the C-terminus of Human MAP3K1 / MEKK1 (NP_005912.1). |
| Conjugation | Un-conjugated |
| Alternate Names | MAPKKK1; SRXY6; MEKK; EC 2.7.11.25; Mitogen-activated protein kinase kinase kinase 1; MEKK1; MAPK/ERK kinase kinase 1; MEKK 1; MEK kinase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A431 | ||||||||
| Observed Size | ~ 160 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13233 Human Mitogen-activated protein kinase kinase kinase 1 Swiss-port # Q62925 Rat Mitogen-activated protein kinase kinase kinase 1 |
|---|---|
| Gene Symbol | MAP3K1 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase |
| Background | The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012] |
| Function | Component of a protein kinase signal transduction cascade (PubMed:9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed:9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed:17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed:9808624). [UniProt] |
| Calculated MW | 164 kDa |
| PTM | Autophosphorylated. [UniProt] |
