ARG43172
anti-MAP3K3 / MEKK3 antibody
anti-MAP3K3 / MEKK3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MAP3K3 / MEKK3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAP3K3 / MEKK3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human MAP3K3 / MEKK3. |
| Conjugation | Un-conjugated |
| Alternate Names | MAPK/ERK kinase kinase 3; MEK kinase 3; EC 2.7.11.25; Mitogen-activated protein kinase kinase kinase 3; MEKK3; MEKK 3; MAPKKK3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa | ||||||||
| Observed Size | ~ 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q61084 Mouse Mitogen-activated protein kinase kinase kinase 3 Swiss-port # Q99759 Human Mitogen-activated protein kinase kinase kinase 3 |
|---|---|
| Gene Symbol | MAP3K3 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 3 |
| Background | This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Function | Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. [UniProt] |
| Calculated MW | 71 kDa |
| PTM | Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity. [UniProt] |
