ARG40410
anti-MARK1 antibody
anti-MARK1 antibody for Western blot and Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MARK1 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MARK1 |
| Antigen Species | Human |
| Immunogen | Fusion protein of Human MARK1. |
| Conjugation | Un-conjugated |
| Alternate Names | PAR1 homolog c; Serine/threonine-protein kinase MARK1; EC 2.7.11.26; MARK; Par1c; Par-1c; EC 2.7.11.1; MAP/microtubule affinity-regulating kinase 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | WB: Mouse heart and brain tissue. | ||||
| Observed Size | ~ 90 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8VHJ5 Mouse Serine/threonine-protein kinase MARK1 |
|---|---|
| Gene Symbol | MARK1 |
| Gene Full Name | MAP/microtubule affinity-regulating kinase 1 |
| Function | Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). [UniProt] |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Appears to localize to an intracellular network. [UniProt] |
| Calculated MW | 89 kDa |
| PTM | Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity (By similarity). Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity. [UniProt] |
