ARG62540

anti-MART1 / Melan A antibody [M2-7C10]

anti-MART1 / Melan A antibody [M2-7C10] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Controls and Markers antibody

Overview

Product Description Mouse Monoclonal antibody [M2-7C10] recognizes MART1 / Melan A
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone M2-7C10
Isotype IgG2b, kappa
Target Name MART1 / Melan A
Immunogen Recombinant hMART-1 protein
Conjugation Un-conjugated
Alternate Names MART-1; MART1; Protein Melan-A; Antigen SK29-AA; Antigen LB39-AA; Melanoma antigen recognized by T-cells 1

Application Instructions

Application Note WB: 0.5-1ug/ml
ELISA: 1-5ug/ml for coating
FACS: 0.5-1ug/million cells
ICC/IF: 1-2ug/ml
IHC-F: 0.5-1ug/ml
IHC-P: 0.5-1ug/ml
IP: 1-2ug/500ug protein lysate
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer PBS (pH 7.4), 1% BSA and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 1% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2315 Human MLANA

Swiss-port # Q16655 Human Melanoma antigen recognized by T-cells 1

Gene Symbol MLANA
Gene Full Name melan-A
Function Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. [UniProt]
Cellular Localization Cytoplasm
Research Area Cancer antibody; Controls and Markers antibody
Calculated MW 13 kDa
PTM Acylated.

Clone References

Hypoxia contributes to melanoma heterogeneity by triggering HIF1α-dependent phenotype switching.

Widmer DS et al.
J Invest Dermatol.,  (2013)

publication_link

 

hr_line

Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells.

WB / Human

Shimizu K et al.
Cancer Res.,  (2013)

publication_link

 

hr_line

A dual role for K63-linked ubiquitin chains in multivesicular body biogenesis and cargo sorting.

WB / 

Erpapazoglou Z et al.
Mol Biol Cell.,  (2012)

publication_link

 

hr_line

The metastatic microenvironment: brain-residing melanoma metastasis and dormant micrometastasis.

Izraely S et al.
Int J Cancer.,  (2012)

publication_link

 

hr_line

The ocular albinism type 1 (OA1) GPCR is ubiquitinated and its traffic requires endosomal sorting complex responsible for transport (ESCRT) function.

ICC/IF / Human

Giordano F et al.
Proc Natl Acad Sci U S A.,  (2011)

publication_link

 

hr_line

Intraglomerular crescentic metastases of malignant melanoma.

IHC / Human

Ozluk Y et al.
Hum Pathol.,  (2011)

publication_link

 

hr_line

Dendritic cell vaccine with mRNA targeted to the proteasome by polyubiquitination.

WB / Primates

Hosoi A et al.
Biochem Biophys Res Commun.,  (2008)

publication_link

 

hr_line

Cyclobutane pyrimidine dimer formation and p53 production in human skin after repeated UV irradiation.

ICC/IF / Human

Yamaguchi Y et al.
Exp Dermatol.,  (2008)

publication_link

 

hr_line

Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.

FACS / Human

Zhang JG et al.
Clin Cancer Res.,  (2007)

publication_link

 

hr_line

MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes.

IP / Human

Hoashi T et al.
J Biol Chem.,  (2005)

publication_link

 

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