ARG55951

anti-MART1 / Melan A antibody [M2-9E3]

anti-MART1 / Melan A antibody [M2-9E3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [M2-9E3] recognizes MART1 / Melan A
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone M2-9E3
Isotype IgG2b, kappa
Target Name MART1 / Melan A
Antigen Species Human
Immunogen Recombinant Human MART-1 protein.
Conjugation Un-conjugated
Alternate Names MART-1; MART1; Protein Melan-A; Antigen SK29-AA; Antigen LB39-AA; Melanoma antigen recognized by T-cells 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2315 Human MLANA

Swiss-port # Q16655 Human Melanoma antigen recognized by T-cells 1

Gene Symbol MLANA
Gene Full Name melan-A
Function Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. [UniProt]
Cellular Localization Cytoplasmic
Calculated MW 13 kDa
PTM Acylated.