ARG40349

anti-MAT1A antibody

anti-MAT1A antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MAT1A
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Specificity The antibody might reacts MAT2A based on sequence homology analysis.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAT1A
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-395 of Human MAT1A (NP_000420.1).
Conjugation Un-conjugated
Alternate Names SAMS1; MAT 1; MAT; Methionine adenosyltransferase I/III; MAT-I/III; Methionine adenosyltransferase 1; MATA1; AdoMet synthase 1; EC 2.5.1.6; SAMS; S-adenosylmethionine synthase isoform type-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat liver
Observed Size 45 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11720 Mouse MAT1A

GeneID: 25331 Rat MAT1A

GeneID: 4143 Human MAT1A

Gene Symbol MAT1A
Gene Full Name methionine adenosyltransferase I, alpha
Background This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]
Function Catalyzes the formation of S-adenosylmethionine from methionine and ATP. [UniProt]
Calculated MW 44 kDa
PTM S-nitrosylation of Cys-120 inactivates the enzyme.

An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond. [UniProt]