ARG59496

anti-MAVS antibody

anti-MAVS antibody for Western blot,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes MAVS
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAVS
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-65 of Human MAVS (NP_065797.2).
Conjugation Un-conjugated
Alternate Names IPS1; IPS-1; Mitochondrial antiviral-signaling protein; Virus-induced-signaling adapter; VISA; CARDIF; Cardif; Interferon beta promoter stimulator protein 1; MAVS; Putative NF-kappa-B-activating protein 031N; CARD adapter inducing interferon beta

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:20 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver and MCF7
Observed Size 70 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 228607 Mouse MAVS

GeneID: 57506 Human MAVS

Swiss-port # Q7Z434 Human Mitochondrial antiviral-signaling protein

Swiss-port # Q8VCF0 Mouse Mitochondrial antiviral-signaling protein

Gene Symbol MAVS
Gene Full Name mitochondrial antiviral signaling protein
Background This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Function Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. [UniProt]
Cellular Localization Mitochondrion outer membrane. Mitochondrion. Peroxisome. [UniProt]
Highlight Related products:
MAVS antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Calculated MW 57 kDa
PTM Ubiquitinated (PubMed:19881509, PubMed:23087404). Undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS/IPS1 proteasomal degradation (PubMed:19881509). Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044). Undergoes 'Lys-48'-linked ubiquitination catalyzed by SMURF1 (PubMed:23087404). [UniProt]