ARG23263
anti-MAdCAM1 antibody [314G8]
anti-MAdCAM1 antibody [314G8] for IHC-Frozen sections,ELISA,Flow cytometry and Human
Overview
| Product Description | Mouse Monoclonal antibody [314G8] recognizes MAdCAM1 Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 314G8 |
| Isotype | IgG1 |
| Target Name | MAdCAM1 |
| Antigen Species | Human |
| Immunogen | Recombinant soluble MAdCAM-1-fc fusion protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Mucosal addressin cell adhesion molecule 1; hMAdCAM-1; MAdCAM-1; MACAM1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13477 Human Mucosal addressin cell adhesion molecule 1 |
|---|---|
| Gene Symbol | MADCAM1 |
| Gene Full Name | mucosal vascular addressin cell adhesion molecule 1 |
| Background | The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. [UniProt] |
| Calculated MW | 40 kDa |
| PTM | The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. [UniProt] |
