ARG42928

anti-MBD4 / MED1 antibody

anti-MBD4 / MED1 antibody for Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MBD4 / MED1
Tested Reactivity Hu, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MBD4 / MED1
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 566-580 of Human MBD4 / MED1. (YHDWLWENHEKLSLS)
Conjugation Un-conjugated
Alternate Names Methyl-CpG-binding endonuclease 1; MED1; Methyl-CpG-binding domain protein 4; Mismatch-specific DNA N-glycosylase; EC 3.2.2.-; Methyl-CpG-binding protein MBD4

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 66 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8930 Human MBD4

Swiss-port # O95243 Human Methyl-CpG-binding domain protein 4

Gene Symbol MBD4
Gene Full Name methyl-CpG binding domain 4 DNA glycosylase
Background The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Function Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 66 kDa