ARG55091

anti-MCM-BP antibody

anti-MCM-BP antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MCM-BP
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Bov
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MCM-BP
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 143-171 (N-terminus) of Human MCM-BP.
Conjugation Un-conjugated
Alternate Names C10orf119; MCM-binding protein; Mini-chromosome maintenance complex-binding protein; MCM-BP

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MDA-MB-231

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79892 Human MCMBP

Swiss-port # Q9BTE3 Human Mini-chromosome maintenance complex-binding protein

Gene Symbol MCMBP
Gene Full Name minichromosome maintenance complex binding protein
Background This gene encodes a protein which is a component of the hexameric minichromosome maintenance (MCM) complex which regulates initiation and elongation of DNA. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion. [UniProt]
Cellular Localization Nucleus. Note=Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 73 kDa