ARG66020

anti-MCP5 / Mast cell chymase antibody (Biotin)

anti-MCP5 / Mast cell chymase antibody (Biotin) for ELISA,Western blot and Mouse

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes MCP5 / Mast cell chymase
Tested Reactivity Ms
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MCP5 / Mast cell chymase
Antigen Species Mouse
Immunogen E. coli derived recombinant Mouse MCP5.
(GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG)
Conjugation Biotin
Alternate Names Chymase; Alpha-chymase; Mast cell protease I; chymase; CYH; EC 3.4.21.39; MCT1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66019 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17228 Mouse CMA1

Swiss-port # P21844 Mouse Chymase

Gene Symbol Cma1
Gene Full Name chymase 1, mast cell
Background This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015]
Function Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. [UniProt]
Calculated MW 27 kDa