ARG22275

anti-MDC1 antibody [P2B11]

anti-MDC1 antibody [P2B11] for Western blot,ICC/IF and Human,Mouse,Bovine,Chimpanzee

Overview

Product Description Mouse Monoclonal antibody [P2B11] recognizes MDC1
Tested Reactivity Hu, Ms, Bov, Chimp
Tested Application ICC/IF, WB
Specificity Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Host Mouse
Clonality Monoclonal
Clone P2B11
Isotype IgG1
Target Name MDC1
Antigen Species Mouse
Immunogen GST-tagged recombinant protein around the N-terminus of Mouse MDC1
Conjugation Un-conjugated
Alternate Names Nuclear factor with BRCT domains 1; Mediator of DNA damage checkpoint protein 1; NFBD1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 240087 Mouse MDC1

GeneID: 9656 Human MDC1

Swiss-port # Q14676 Human Mediator of DNA damage checkpoint protein 1

Swiss-port # Q5PSV9 Mouse Mediator of DNA damage checkpoint protein 1

Gene Symbol Mdc1
Gene Full Name mediator of DNA damage checkpoint 1
Background The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]
Function Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. [UniProt]
Cellular Localization Nucleus
Calculated MW 227 kDa
PTM Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain.
Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation.
Ubiquitinated by RNF4, leading to proteasomal degradation; undergoes 'Lys-48'-linked polyubiquitination.