ARG43423

anti-MED1 / TRAP220 antibody

anti-MED1 / TRAP220 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MED1 / TRAP220
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MED1 / TRAP220
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 664-720 of Human MED1 / TRAP220.
Conjugation Un-conjugated
Alternate Names TR-interacting protein 2; TRIP-2; Mediator of RNA polymerase II transcription subunit 1; DRIP230; PPAR-binding protein; Vitamin D receptor-interacting protein complex component DRIP205; Peroxisome proliferator-activated receptor-binding protein; Trap220; DRIP205; CRSP1; TRAP220; PBP; p53 regulatory protein RB18A; CRSP200; Mediator complex subunit 1; Thyroid receptor-interacting protein 2; PPARBP; TRIP2; PPARGBP; ARC205; Thyroid hormone receptor-associated protein complex 220 kDa component; RB18A; Activator-recruited cofactor 205 kDa component

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A549, HEK293T, PC12 and CT26
Observed Size ~ 250 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.2), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19014 Mouse MED1

GeneID: 5469 Human MED1

Swiss-port # Q15648 Human Mediator of RNA polymerase II transcription subunit 1

Swiss-port # Q925J9 Mouse Mediator of RNA polymerase II transcription subunit 1

Gene Symbol MED1
Gene Full Name mediator complex subunit 1
Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). [UniProt]
Cellular Localization Nucleus. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3. [UniProt]
Calculated MW 168 kDa
PTM Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. [UniProt]