ARG55387

anti-MED12 antibody

anti-MED12 antibody for Western blot and Human,Mouse

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MED12
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MED12
Antigen Species Mouse
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1991-2025 (C-terminus) of Mouse MED12.
Conjugation Un-conjugated
Alternate Names ARC240; OKS; TNRC11; Thyroid hormone receptor-associated protein complex 230 kDa component; TRAP230; OPA-containing protein; OPA1; Mediator complex subunit 12; MED12S; FGS1; CAGH45; Mediator of RNA polymerase II transcription subunit 12; Activator-recruited cofactor 240 kDa component; Trinucleotide repeat-containing gene 11 protein; OHDOX; HOPA; CAG repeat protein 45; Trap230

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 59024 Mouse MED12

GeneID: 9968 Human MED12

Swiss-port # A2AGH6 Mouse Mediator of RNA polymerase II transcription subunit 12

Swiss-port # Q93074 Human Mediator of RNA polymerase II transcription subunit 12

Gene Symbol Med12
Gene Full Name mediator complex subunit 12
Background The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity). [UniProt]
Research Area Gene Regulation antibody
Calculated MW 243 kDa