ARG59176

anti-MED14 antibody

anti-MED14 antibody for Flow cytometry,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes MED14
Tested Reactivity Hu, Ms
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MED14
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 586-615 of Human MED14.
Conjugation Un-conjugated
Alternate Names CSRP; Transcriptional coactivator CRSP150; TRAP170; RGR1; Mediator complex subunit 14; Thyroid hormone receptor-associated protein complex 170 kDa component; CRSP complex subunit 2; CXorf4; Vitamin D3 receptor-interacting protein complex 150 kDa component; DRIP150; CRSP150; ARC150; CRSP2; EXLM1; RGR1 homolog; Trap170; hRGR1; Activator-recruited cofactor 150 kDa component; Cofactor required for Sp1 transcriptional activation subunit 2; Mediator of RNA polymerase II transcription subunit 14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control DU145

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26896 Mouse MED14

GeneID: 9282 Human MED14

Swiss-port # A2ABV5 Mouse Mediator of RNA polymerase II transcription subunit 14

Swiss-port # O60244 Human Mediator of RNA polymerase II transcription subunit 14

Gene Symbol MED14
Gene Full Name mediator complex subunit 14
Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 161 kDa