ARG43231

anti-MED15 / PCQAP antibody [6F4]

anti-MED15 / PCQAP antibody [6F4] for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [6F4] recognizes MED15 / PCQAP
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 6F4
Isotype IgG1
Target Name MED15 / PCQAP
Antigen Species Human
Immunogen Recombinant protein corresponding to M1-A285 of Human MED15 / PCQAP.
Conjugation Un-conjugated
Alternate Names Activator-recruited cofactor 105 kDa component; PC2 glutamine/Q-rich-associated protein; Mediator complex subunit 15; TPA-inducible gene 1 protein; ARC105; Positive cofactor 2 glutamine/Q-rich-associated protein; CTG7A; Trinucleotide repeat-containing gene 7 protein; Mediator of RNA polymerase II transcription subunit 15; CTG repeat protein 7a; CAG7A; TIG-1; TIG1; PCQAP; TNRC7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 87 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51586 Human MED15

Swiss-port # Q96RN5 Human Mediator of RNA polymerase II transcription subunit 15

Gene Symbol MED15
Gene Full Name mediator complex subunit 15
Background The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. [UniProt]
Cellular Localization Cytoplasm. Nucleus. [UniProt]
Calculated MW 87 kDa
PTM Ubiquitinated by TRIM11, leading to proteasomal degradation. [UniProt]