ARG59207

anti-MED20 antibody

anti-MED20 antibody for Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MED20
Tested Reactivity Hu, Rat
Predict Reactivity Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MED20
Antigen Species Human
Immunogen Recombinant protein corresponding to M1-R212 of Human MED20.
Conjugation Un-conjugated
Alternate Names Mediator complex subunit 20; PRO0213; TRFP; hTRFP; Mediator of RNA polymerase II transcription subunit 20; TRF-proximal protein homolog

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 316209 Rat MED20

GeneID: 9477 Human MED20

Swiss-port # Q5XIE9 Rat Mediator of RNA polymerase II transcription subunit 20

Swiss-port # Q9H944 Human Mediator of RNA polymerase II transcription subunit 20

Gene Symbol MED20
Gene Full Name mediator complex subunit 20
Background This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 23 kDa