ARG45118
anti-MED23 antibody
anti-MED23 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED23 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED23 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | MED23; Mediator Complex Subunit 23; DRIP130; CRSP130; CRSP3; Sur2; Cofactor Required For Sp1 Transcriptional Activation Subunit 3; Mediator Of RNA Polymerase II Transcription Subunit 23; Activator-Recruited Cofactor 130 KDa Component; ARC130; MRT18; Cofactor Required For Sp1 Transcriptional Activation, Subunit 3, 130kDa; Vitamin D3 Receptor-Interacting Protein Complex 130 KDa Component; Mental Retardation, Non-Syndromic, Autosomal Recessive, 18; Vitamin D3 Receptor Interacting Protein; 130 KDa Transcriptional Co-Activator; 133 KDa Transcriptional Co-Activator; Transcriptional Coactivator CRSP130; CRSP Complex Subunit 3; Protein Sur-2 Homolog; KIAA1216; CRSP133; HSur-2; SUR-2; SUR2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q80YQ2 Mouse Mediator of RNA polymerase II transcription subunit 23 Swiss-port # Q9ULK4 Human Mediator of RNA polymerase II transcription subunit 23 |
|---|---|
| Gene Symbol | MED23 |
| Gene Full Name | Mediator Complex Subunit 23 |
| Background | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling. [Unuprot] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 156 kDa |
