ARG52336

anti-MEK5 phospho (Ser311 / Thr315) antibody

anti-MEK5 phospho (Ser311 / Thr315) antibody for Western blot and Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MEK5 phospho (Ser311 / Thr315)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis, Zfsh
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MEK5
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH
Conjugation Un-conjugated
Alternate Names MEK 5; HsT17454; MAPK/ERK kinase 5; PRKMK5; EC 2.7.12.2; MAPKK 5; MEK5; MAPKK5; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29568 Rat MAP2K5

Swiss-port # Q62862 Rat Dual specificity mitogen-activated protein kinase kinase 5

Gene Symbol MAP2K5
Gene Full Name mitogen activated protein kinase kinase 5
Background MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 50 kDa
PTM Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.