ARG63353

anti-MEL18 / PCGF2 antibody

anti-MEL18 / PCGF2 antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes MEL18 / PCGF2
Tested Reactivity Hu
Predict Reactivity Cow, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MEL18 / PCGF2
Antigen Species Human
Immunogen C-KMTVNGAPVPPLT
Conjugation Un-conjugated
Alternate Names RING finger protein 110; Polycomb group RING finger protein 2; ZNF144; MEL-18; RNF110; Zinc finger protein 144; DNA-binding protein Mel-18

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7703 Human PCGF2

Swiss-port # P35227 Human Polycomb group RING finger protein 2

Background The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
Calculated MW 38 kDa
PTM Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers (By similarity).