ARG44512

anti-METTL18 antibody

anti-METTL18 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes METTL18
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name METTL18
Antigen Species Human
Immunogen Human METTL18 recombinant protein
Conjugation Un-conjugated
Alternate Names METTL18; Methyltransferase 18, RPL3 N3(Tau)-Histidine; AsTP2; Methyltransferase Like 18 ; C1orf156; HPM1; Histidine Protein Methyltransferase 1 Homolog; Methyltransferase 18, RPL3 N3-Histidine; Methyltransferase-Like Protein 18; Arsenic-Transactivated Protein 2; MGC9084; Histidine Protein Methyltransferase 1; Chromosome 1 Open Reading Frame 156

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 92342 Human METTL18

Swiss-port # O95568 Human Histidine protein methyltransferase 1 homolog

Gene Symbol METTL18
Gene Full Name Methyltransferase 18, RPL3 N3(Tau)-Histidine
Background Enables heat shock protein binding activity and protein-L-histidine N-tele-methyltransferase activity. Involved in peptidyl-lysine monomethylation; regulation of gene expression; and regulation of ribosome biogenesis. Located in cytosol and nucleolus. Part of protein-containing complex.
Function Protein-L-histidine N-tele-methyltransferase that specifically monomethylates RPL3, thereby regulating translation elongation.
Cellular Localization Cytoplasm, Nucleus
Calculated MW 42 kDa
PTM Methylation, Phosphoprotein

Images (5) Click the Picture to Zoom In

  • ARG44512 anti-METTL18 antibody IHC-P image

    Immunohistochemistry: Human colon adenocarcinoma stained with ARG44512 anti-METTL18 antibody at 2 μg/mL dilution.

  • ARG44512 anti-METTL18 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG44512 anti-METTL18 antibody at 5 μg/mL dilution.

  • ARG44512 anti-METTL18 antibody WB image

    Western blot: A431 and PC-3 stained with ARG44512 anti-METTL18 antibody at 0.5 μg/mL dilution.

  • ARG44512 anti-METTL18 antibody FACS image

    Flow Cytometry: A431 stained with ARG44512 anti-METTL18 antibody at 1 μg/10^6 cells dilution.

  • ARG44512 anti-METTL18 antibody IHC-P image

    Immunohistochemistry: Human placenta stained with ARG44512 anti-METTL18 antibody at 2 μg/mL dilution.