ARG57980

anti-MFGE8 / Lactadherin antibody

anti-MFGE8 / Lactadherin antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MFGE8 / Lactadherin
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MFGE8 / Lactadherin
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-200 of Human MFG-E8 (NP_001108086.1).
Conjugation Un-conjugated
Alternate Names SPAG10; MFGM; HsT19888; HMFG; Milk fat globule-EGF factor 8; MFG-E8; BA46; hP47; EDIL1; Lactadherin; OAcGD3S; Breast epithelial antigen BA46; SED1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart
Observed Size ~ 53 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17304 Mouse MFGE8

GeneID: 25277 Rat MFGE8

Swiss-port # P21956 Mouse Lactadherin

Swiss-port # P70490 Rat Lactadherin

Gene Symbol MFGE8
Gene Full Name milk fat globule-EGF factor 8 protein
Background This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Function Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.

Medin is the main constituent of aortic medial amyloid. [UniProt]
Cellular Localization Membrane, Peripheral membrane protein, Secreted. [UniProt]
Calculated MW 43 kDa
PTM Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. [UniProt]