ARG56051
anti-MFGE8 / Lactadherin antibody [SPM291]
anti-MFGE8 / Lactadherin antibody [SPM291] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
Product Description | Mouse Monoclonal antibody [SPM291] recognizes MFGE8 / Lactadherin |
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Tested Reactivity | Hu |
Tested Application | IHC-P |
Host | Mouse |
Clonality | Monoclonal |
Clone | SPM291 |
Isotype | IgG1, kappa |
Target Name | MFGE8 / Lactadherin |
Antigen Species | Human |
Immunogen | A Human milk fat globule membrane preparation. |
Conjugation | Un-conjugated |
Alternate Names | SPAG10; MFGM; HsT19888; HMFG; Milk fat globule-EGF factor 8; MFG-E8; BA46; hP47; EDIL1; Lactadherin; OAcGD3S; Breast epithelial antigen BA46; SED1 |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
Preservative | 0.05% Sodium azide |
Stabilizer | 0.1 mg/ml BSA |
Concentration | 0.2 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | MFGE8 |
Gene Full Name | milk fat globule-EGF factor 8 protein |
Background | This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
Function | Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid. [UniProt] |
Cellular Localization | Cell surface and cytoplasmic |
Calculated MW | 43 kDa |
PTM | Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. |