ARG40462

anti-MGAT5 antibody

anti-MGAT5 antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MGAT5
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MGAT5
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 627-741 of Human MGAT5 (NP_002401.1).
Conjugation Un-conjugated
Alternate Names EC 2.4.1.155; Mannoside acetylglucosaminyltransferase 5; GlcNAc-T V; GNT-VA; GNT-V; Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; N-acetylglucosaminyl-transferase V; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size 115 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4249 Human MGAT5

Swiss-port # Q09328 Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Gene Symbol MGAT5
Gene Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Background The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]
Function Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. [UniProt]
Cellular Localization Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt]
Calculated MW 85 kDa