ARG41162
anti-MGEA5 / OGA antibody
anti-MGEA5 / OGA antibody for Western blot and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes MGEA5 / OGA |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | MGEA5 / OGA |
Antigen Species | Human |
Immunogen | Recombinant fusion protein corresponding to aa. 380-580 of Human MGEA5 (NP_001135906.1). |
Conjugation | Un-conjugated |
Alternate Names | Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; N-acetyl-beta-D-glucosaminidase; MEA5; NCOAT; EC 3.2.1.-; Meningioma-expressed antigen 5; Beta-N-acetylhexosaminidase; Beta-hexosaminidase; EC 3.2.1.169; Protein O-GlcNAcase; Beta-N-acetylglucosaminidase; OGA; N-acetyl-beta-glucosaminidase |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
Positive Control | Mouse brain | ||||
Observed Size | ~120-130 kda |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | MGEA5 |
Gene Full Name | meningioma expressed antigen 5 (hyaluronidase) |
Background | The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008] |
Function | Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. [UniProt] |
Cellular Localization | Isoform 3: Nucleus. Isoform 1: Cytoplasm. [UniProt] |
Calculated MW | 103 kDa |
PTM | Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity. [UniProt] |