ARG41162

anti-MGEA5 / OGA antibody

anti-MGEA5 / OGA antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MGEA5 / OGA
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MGEA5 / OGA
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 380-580 of Human MGEA5 (NP_001135906.1).
Conjugation Un-conjugated
Alternate Names Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; N-acetyl-beta-D-glucosaminidase; MEA5; NCOAT; EC 3.2.1.-; Meningioma-expressed antigen 5; Beta-N-acetylhexosaminidase; Beta-hexosaminidase; EC 3.2.1.169; Protein O-GlcNAcase; Beta-N-acetylglucosaminidase; OGA; N-acetyl-beta-glucosaminidase

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:200 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain
Observed Size ~120-130 kda

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10724 Human MGEA5

GeneID: 154968 Rat MGEA5

GeneID: 76055 Mouse MGEA5

Gene Symbol MGEA5
Gene Full Name meningioma expressed antigen 5 (hyaluronidase)
Background The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Function Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. [UniProt]
Cellular Localization Isoform 3: Nucleus. Isoform 1: Cytoplasm. [UniProt]
Calculated MW 103 kDa
PTM Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity. [UniProt]