ARG44144
anti-MGME1 antibody
anti-MGME1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Disease variant, Primary mitochondrial disease, Progressive external ophthalmoplegia
Overview
| Product Description | Rabbit Polyclonal recognizes MGME1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MGME1 |
| Antigen Species | Human |
| Immunogen | Human MGME1 recombinant protein (Position: K4-D310). |
| Conjugation | Un-conjugated |
| Alternate Names | MGME1; Mitochondrial Genome Maintenance Exonuclease 1; DDK1; BA504H3.4; C20orf72; Chromosome 20 Open Reading Frame 72; EC 3.1.-.-; MTDPS11 |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BQP7 Human Mitochondrial genome maintenance exonuclease 1 |
|---|---|
| Gene Symbol | MGME1 |
| Gene Full Name | Mitochondrial Genome Maintenance Exonuclease 1 |
| Background | The protein encoded by this gene is a nuclear-encoded mitochondrial protein necessary for the maintenance of mitochondrial genome synthesis. The encoded protein is a RecB-type exonuclease and primarily cleaves single-stranded DNA. Defects in this gene have been associated with mitochondrial DNA depletion syndrome-11. Three transcript variants encoding different isoforms have been found for this gene. |
| Function | Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells. |
| Cellular Localization | Mitochondrion |
| Research Area | Disease variant, Primary mitochondrial disease, Progressive external ophthalmoplegia |
| Calculated MW | 39 kDa |
| PTM | Phosphoprotein |
