ARG22781
anti-MHC Class I RT1A antibody [OX-18]
anti-MHC Class I RT1A antibody [OX-18] for ELISA,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Rat
Overview
| Product Description | Mouse Monoclonal antibody [OX-18] recognizes MHC Class I RT1A This antibody recognizes a monomorphic determinant of Rat MHC Class I (RT1A), expressed by all Rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized. Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 has been used in immunoaffinity purification of Rat MHC class I molecules (Fukumoto et al. 1982). Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 is routinely tested in flow cytometry on Rat splenocytes. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA, FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | OX-18 |
| Isotype | IgG1 |
| Target Name | MHC Class I RT1A |
| Antigen Species | Rat |
| Immunogen | Rat spleen cell glycoproteins. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
