ARG23530
anti-MHC Class I antibody [2G5] (FITC)
anti-MHC Class I antibody [2G5] (FITC) for Flow cytometry and Human,Mouse,Rat,Bovine,Guinea pig,Hamster,Pig,Sheep
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [2G5] recognizes MHC Class I. Mouse anti Mouse MHC Class I antibody, clone 2G5 recognizes a monomorphic epitope present on murine MHC class I molecules, expressed at varying levels on the majority of nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved (Claesson et al. 1994). Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class I. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Gpig, Hm, Pig, Sheep |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2G5 |
| Isotype | IgG2b |
| Target Name | MHC Class I |
| Antigen Species | Mouse |
| Immunogen | Purified H-2Kb and H-2Db MHC-I molecules. |
| Conjugation | FITC |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
