ARG22708
anti-MHC Class II (Monomorphic) antibody [YKIX334.2]
anti-MHC Class II (Monomorphic) antibody [YKIX334.2] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Dog
Overview
| Product Description | Rat Monoclonal antibody [YKIX334.2] recognizes MHC Class II (Monomorphic) This antibody recognizes a monomorphic epitope on Canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994). The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells. |
|---|---|
| Tested Reactivity | Dog |
| Tested Application | FACS, IHC-Fr, IP |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YKIX334.2 |
| Isotype | IgG2a |
| Target Name | MHC Class II (Monomorphic) |
| Antigen Species | Dog |
| Immunogen | Con A activated canine peripheral blood cells. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
