ARG22771
anti-MHC Class II RT1B antibody [OX-6] (FITC)
anti-MHC Class II RT1B antibody [OX-6] (FITC) for Flow cytometry and Mouse,Rat
Overview
Product Description | FITC-conjugated Mouse Monoclonal antibody [OX-6] recognizes MHC Class II RT1B This antibody recognizes a monomorphic determinant of the Rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Rat MHC Class II RT1B antibody, clone OX-6 does not react with the Rat BDIX strain due to a defect in RT1B expression (Male et al. 1987). The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987). This product is routinely tested in flow cytometry on Rat splenocytes. |
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Tested Reactivity | Ms, Rat |
Tested Application | FACS |
Host | Mouse |
Clonality | Monoclonal |
Clone | OX-6 |
Isotype | IgG1 |
Target Name | MHC Class II RT1B |
Antigen Species | Rat |
Immunogen | Rat thymocyte membrane glycoproteins. |
Conjugation | FITC |
Application Instructions
Application Suggestion |
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Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS, 0.09% Sodium azide and 1% BSA. |
Preservative | 0.09% Sodium azide |
Stabilizer | 1% BSA |
Concentration | 0.1 mg/ml |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |