ARG63071

anti-MHC Class II antibody [M5/114] (FITC)

anti-MHC Class II antibody [M5/114] (FITC) for Flow cytometry and Mouse

Immune System antibody

Overview

Product Description

FITC-conjugated Rat Monoclonal antibody [M5/114] recognizes MHC Class II

Tested Reactivity Ms
Tested Application FACS
Specificity The clone M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Host Rat
Clonality Monoclonal
Clone M5/114
Isotype IgG2b
Target Name MHC Class II
Antigen Species Mouse
Immunogen Activated C57BL/6 mouse spleen cells.
Conjugation FITC
Alternate Names HLA-DRB; HLA class II histocompatibility antigen, DRB1-3 chain; SS1; MHC class II antigen DRB1*3; HLA-DR1B; DRw10; Clone P2-beta-3; DRB1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol H2-D1
Gene Full Name histocompatibility 2, D region locus 1
Background MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]
Research Area Immune System antibody
Calculated MW 30 kDa
PTM Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II.