ARG41742
anti-MICAL1 antibody
anti-MICAL1 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MICAL1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MICAL1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 718-1067 of Human MICAL1 (NP_073602.3). |
| Conjugation | Un-conjugated |
| Alternate Names | MICAL; EC 1.14.13.-; Protein-methionine sulfoxide oxidase MICAL1; NICAL; MICAL-1; NEDD9-interacting protein with calponin homology and LIM domains; Molecule interacting with CasL protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | A549 | ||||
| Observed Size | ~ 132 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8TDZ2 Human Protein-methionine sulfoxide oxidase MICAL1 |
|---|---|
| Gene Symbol | MICAL1 |
| Gene Full Name | microtubule associated monooxygenase, calponin and LIM domain containing 1 |
| Background | This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
| Function | Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (Probable). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. [UniProt] |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Midbody. Note=Accumulates transiently at the abscission site before abscission occurs. [UniProt] |
| Calculated MW | 118 kDa |
