ARG41742

anti-MICAL1 antibody

anti-MICAL1 antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MICAL1
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MICAL1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 718-1067 of Human MICAL1 (NP_073602.3).
Conjugation Un-conjugated
Alternate Names MICAL; EC 1.14.13.-; Protein-methionine sulfoxide oxidase MICAL1; NICAL; MICAL-1; NEDD9-interacting protein with calponin homology and LIM domains; Molecule interacting with CasL protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:200 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A549
Observed Size ~ 132 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64780 Human MICAL1

Swiss-port # Q8TDZ2 Human Protein-methionine sulfoxide oxidase MICAL1

Gene Symbol MICAL1
Gene Full Name microtubule associated monooxygenase, calponin and LIM domain containing 1
Background This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
Function Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (Probable). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. [UniProt]
Cellular Localization Cytoplasm. Cytoplasm, cytoskeleton. Midbody. Note=Accumulates transiently at the abscission site before abscission occurs. [UniProt]
Calculated MW 118 kDa