ARG40448
anti-MID1 antibody
anti-MID1 antibody for Western blot,ICC/IF and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MID1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MID1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 478-667 of Human MID1 (NP_000372.1). |
| Conjugation | Un-conjugated |
| Alternate Names | OSX; MIDIN; RING finger protein Midline-1; FXY; EC 6.3.2.-; E3 ubiquitin-protein ligase Midline-1; RNF59; Midin; TRIM18; Tripartite motif-containing protein 18; XPRF; ZNFXY; RING finger protein 59; GBBB1; OGS1; BBBG1; Putative transcription factor XPRF; OS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse heart | ||||||
| Observed Size | 85 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15344 Human E3 ubiquitin-protein ligase Midline-1 Swiss-port # O70583 Mouse E3 ubiquitin-protein ligase Midline-1 |
|---|---|
| Gene Symbol | MID1 |
| Gene Full Name | midline 1 |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined. [provided by RefSeq, Jul 2010] |
| Function | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. [UniProt] |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis. [UniProt] |
| Calculated MW | 75 kDa |
| PTM | Phosphorylated on serine and threonine residues. [UniProt] |
