ARG66709

anti-MMP1 antibody

anti-MMP1 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MMP1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MMP1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within the C-terminal region of Human MMP1.
Conjugation Un-conjugated
Alternate Names MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:500
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 54 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol MMP1
Gene Full Name matrix metallopeptidase 1
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 54 kDa
PTM Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt]