ARG40901

anti-MMP12 antibody

anti-MMP12 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MMP12
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MMP12
Antigen Species Human
Immunogen Synthetic peptide derived from Human MMP12.
Conjugation Un-conjugated
Alternate Names ME; EC 3.4.24.65; Macrophage metalloelastase; MMP-12; hME; MME; HME; Matrix metalloproteinase-12; Macrophage elastase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
IP1:30
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WI-38

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117033 Rat MMP12

GeneID: 17381 Mouse MMP12

GeneID: 4321 Human MMP12

Gene Symbol MMP12
Gene Full Name matrix metallopeptidase 12
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Function May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 54 kDa