ARG40613

anti-MMP9 antibody

anti-MMP9 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Brain Injury IHC Study antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes MMP9
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MMP9
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 538-707 of Human MMP9 (NP_004985.2).
Conjugation Un-conjugated
Alternate Names Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control B cells
Observed Size 82 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17395 Mouse MMP9

GeneID: 4318 Human MMP9

GeneID: 81687 Rat MMP9

Gene Symbol MMP9
Gene Full Name matrix metallopeptidase 9
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Research Area Brain Injury IHC Study antibody
Calculated MW 78 kDa
PTM Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.

N- and O-glycosylated. [UniProt]

Specific References

Effects of STAT3 on aging-dependent neovascularization impairment following limb ischemia: from bedside to bench

WB / Mouse

Wei-Ting Chang et al.
AGING,  (2022)

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