ARG59958
anti-MOCS3 antibody
anti-MOCS3 antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MOCS3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MOCS3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 271-460 of Human MOCS3 (NP_055299.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Sulfur carrier protein MOCS2A adenylyltransferase; Adenylyltransferase and sulfurtransferase MOCS3; Adenylyltransferase MOCS3; EC 2.8.1.11; UBA4; EC 2.7.7.80; MPT synthase sulfurylase; Molybdopterin synthase sulfurylase; Sulfur carrier protein MOCS2A sulfurtransferase; Molybdenum cofactor synthesis protein 3; Sulfurtransferase MOCS3 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Mouse ovary and HepG2 | ||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # A2BDX3 Mouse Adenylyltransferase and sulfurtransferase MOCS3 Swiss-port # O95396 Human Adenylyltransferase and sulfurtransferase MOCS3 |
|---|---|
| Gene Symbol | MOCS3 |
| Gene Full Name | molybdenum cofactor synthesis 3 |
| Background | Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012] |
| Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 50 kDa |
Images (1) Click the Picture to Zoom In
-
ARG59958 anti-MOCS3 antibody WB image
Western blot: 25 µg of Mouse ovary and HepG2 cell lysates stained with ARG59958 anti-MOCS3 antibody at 1:1000 dilution.
