ARG53344

anti-MPO / Myeloperoxidase antibody [SP72]

anti-MPO / Myeloperoxidase antibody [SP72] for IHC-Formalin-fixed paraffin-embedded sections and Human

Inflammatory Cell Marker antibody; Neurophil Marker antibody

Overview

Product Description Rabbit Monoclonal antibody [SP72] recognizes MPO / Myeloperoxidase
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP72
Isotype IgG
Target Name MPO / Myeloperoxidase
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of human myeloperoxidase protein.
Conjugation Un-conjugated
Alternate Names MPO; Myeloperoxidase; EC 1.11.2.2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4353 Human MPO

Swiss-port # P05164 Human Myeloperoxidase

Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Function Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt]
Cellular Localization Cytoplasm
Highlight Related products:
MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Inflammatory Cell Marker antibody; Neurophil Marker antibody
Calculated MW 84 kDa