ARG53344
anti-MPO / Myeloperoxidase antibody [SP72]
anti-MPO / Myeloperoxidase antibody [SP72] for IHC-Formalin-fixed paraffin-embedded sections and Human
Inflammatory Cell Marker antibody; Neurophil Marker antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP72] recognizes MPO / Myeloperoxidase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP72 |
| Isotype | IgG |
| Target Name | MPO / Myeloperoxidase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminus of human myeloperoxidase protein. |
| Conjugation | Un-conjugated |
| Alternate Names | MPO; Myeloperoxidase; EC 1.11.2.2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Function | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| Cellular Localization | Cytoplasm |
| Highlight | Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Inflammatory Cell Marker antibody; Neurophil Marker antibody |
| Calculated MW | 84 kDa |
