ARG43749

anti-MPO / Myeloperoxidase antibody [rMPO/6904]

anti-MPO / Myeloperoxidase antibody [rMPO/6904] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Inflammatory Cell Marker antibody; Neurophil Marker antibody

Overview

Product Description Recombinant Mouse Monoclonal antibody [rMPO/6904] recognizes MPO / Myeloperoxidase
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone rMPO/6904
Isotype IgG1
Target Name MPO / Myeloperoxidase
Antigen Species Human
Immunogen Synthetic peptide within aa. 150-250 of Human MPO / Myeloperoxidase.
Conjugation Un-conjugated
Protein Full Name Myeloperoxidase
Alternate Names MPO; Myeloperoxidase; EC 1.11.2.2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 μg/ml
WB1 - 5 μg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human spleen; bone marrow
Observed Size 84 kDa

Properties

Form Liquid
Purification Purification with Protein A/G affinity.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4353 Human MPO

Swiss-port # P05164 Human Myeloperoxidase

Gene Symbol MPO
Gene Full Name myeloperoxidase
Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Function Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt]
Cellular Localization Lysosome. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30197 MPO ELISA Antibody Duo
Related products:
MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Inflammatory Cell Marker antibody; Neurophil Marker antibody
Calculated MW 60 kDa, 84 kDa, 89 kDa