ARG40105

anti-MRPS35 antibody

anti-MRPS35 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes MRPS35
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MRPS35
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-323 of Human MRPS35 (NP_068593.2).
Conjugation Un-conjugated
Alternate Names HDCMD11P; S28mt; MRPS28; MRP-S35; MDS023; 28S ribosomal protein S35, mitochondrial; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney and HT-29
Observed Size ~ 38 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 232536 Mouse MRPS35

GeneID: 60488 Human MRPS35

Swiss-port # P82673 Human 28S ribosomal protein S35, mitochondrial

Swiss-port # Q8BJZ4 Mouse 28S ribosomal protein S35, mitochondrial

Gene Symbol MRPS35
Gene Full Name mitochondrial ribosomal protein S35
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010]
Cellular Localization Mitochondrion. [UniProt]
Calculated MW 37 kDa