ARG58057

anti-MSH2 antibody

anti-MSH2 antibody for Flow cytometry,Western blot and Human

Cancer antibody; Gene Regulation antibody; DNA Mismatch Repair System Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MSH2
Tested Reactivity Hu
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MSH2
Antigen Species Human
Immunogen Synthetic peptide derived from Human MSH2.
Conjugation Un-conjugated
Alternate Names DNA mismatch repair protein Msh2; COCA1; HNPCC1; FCC1; LCFS2; MutS protein homolog 2; hMSH2; HNPCC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4436 Human MSH2

Swiss-port # P43246 Human DNA mismatch repair protein Msh2

Gene Symbol MSH2
Gene Full Name mutS homolog 2
Background This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Function Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. [UniProt]
Cellular Localization Nucleus. [UniProt]
Research Area Cancer antibody; Gene Regulation antibody; DNA Mismatch Repair System Study antibody
Calculated MW 105 kDa
PTM Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway. [UniProt]