ARG43105

anti-MTDH / AEG1 antibody

anti-MTDH / AEG1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody recognizes MTDH / AEG1
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name MTDH / AEG1
Antigen Species Human
Immunogen Purified recombinant fragment of Human MTDH / AEG1.
Conjugation Un-conjugated
Alternate Names 3D3/LYRIC; AEG-1; Metastasis adhesion protein; 3D3; LYRIC/3D3; AEG1; Protein LYRIC; Lysine-rich CEACAM1 co-isolated protein; LYRIC; Metadherin; Astrocyte elevated gene-1 protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:400
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7
Observed Size ~ 73 kDa

Properties

Form Liquid
Purification Unpurified
Buffer Ascitic fluid and 0.03% Sodium azide.
Preservative 0.03% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 92140 Human MTDH

Swiss-port # Q86UE4 Human Protein LYRIC

Gene Symbol MTDH
Gene Full Name metadherin
Function Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. [UniProt]
Calculated MW 64 kDa