ARG57149
anti-MTH1 antibody [3B3]
anti-MTH1 antibody [3B3] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [3B3] recognizes MTH1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3B3 |
| Isotype | IgG1, kappa |
| Target Name | MTH1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-156 of Human MTH1 |
| Conjugation | Un-conjugated |
| Alternate Names | 8-oxo-dGTPase; MTH1; EC 3.6.1.56; EC 3.6.1.55; 7,8-dihydro-8-oxoguanine triphosphatase; Nudix motif 1; 2-hydroxy-dATP diphosphatase; Nucleoside diphosphate-linked moiety X motif 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P36639 Human 7,8-dihydro-8-oxoguanine triphosphatase |
|---|---|
| Gene Symbol | NUDT1 |
| Gene Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 1 |
| Background | Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008] |
| Function | Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA. [UniProt] |
| Calculated MW | 23 kDa |
| PTM | The N-terminus is blocked. |
