ARG42226

anti-MTHFD1L antibody

anti-MTHFD1L antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes MTHFD1L
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog, Pig
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MTHFD1L
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human MTHFD1L. (C-DTNDRFLRKITIGQ) (NP_056255.2)
Conjugation Un-conjugated
Alternate Names MTC1THFS; dJ292B18.2; Formyltetrahydrofolate synthetase; EC 6.3.4.3; Monofunctional C1-tetrahydrofolate synthase, mitochondrial; FTHFSDC1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 110 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25902 Human MTHFD1L

GeneID: 270685 Mouse MTHFD1L

Swiss-port # Q3V3R1 Mouse Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Swiss-port # Q6UB35 Human Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Gene Symbol MTHFD1L
Gene Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Background The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Function May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. [UniProt]
Cellular Localization Mitochondrion. [UniProt]
Calculated MW 106 kDa