ARG66202

anti-MUC16 / CA125 antibody [SQab1724]

anti-MUC16 / CA125 antibody [SQab1724] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1724] recognizes MUC16 / CA125
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1724
Target Name MUC16 / CA125
Antigen Species Human
Immunogen Synthetic peptide around aa. 12200‐12300 of MUC16 / CA125.
Conjugation Un-conjugated
Alternate Names Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:800
IHC-P1:200 ‐ 1:400
WB1:500
Application Note IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 94025 Human MUC16

Swiss-port # Q8WXI7 Human Mucin-16

Gene Symbol MUC16
Gene Full Name mucin 16, cell surface associated
Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt]
Highlight Related products:
MUC16 antibodies; MUC16 ELISA Kits;
Related news:
Cancer Pathology Markers (SQ clones)
Calculated MW 1519 kDa
PTM Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

Customer's Feedback

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Review for anti-MUC16 / CA125 antibody [SQab1724]

Application:WB

Sample:HeLa and A431

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC