ARG10156
anti-MUC16 / CA125 antibody [X75]
anti-MUC16 / CA125 antibody [X75] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [X75] recognizes MUC16 / CA125 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | X75 |
| Isotype | IgG1 |
| Target Name | MUC16 / CA125 |
| Antigen Species | Human |
| Immunogen | MUC16 / CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa). |
| Epitope | Epitope specificity group B (ISOBM classification) similar to M-11. |
| Conjugation | Un-conjugated |
| Alternate Names | Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | Sandwich ELISA (Capture antibody - Detection antibody): ARG10156 - ARG10351 ARG10351 - ARG10156 ARG10534 - ARG10156 IHC-P: Antigen Retrieval: Heat mediation was performed. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ion exchange chromatography. |
| Buffer | 10 mM Tris (pH 7.5), 0.15 M NaCl and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Concentration | 1.0-2.0 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MUC16 |
| Gene Full Name | mucin 16, cell surface associated |
| Function | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt] |
| Research Area | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 1519 kDa |
| PTM | Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
Clone References
New construction of an animal model for the orthotopic transplantation of an ovarian tumor.
IHC-P, FACS /
The use of ovarian cancer cells from patients undergoing surgery to generate primary cultures capable of undergoing functional analysis.
ICC/IF / Human
Novel treatment option for MUC16-positive malignancies with the targeted TRAIL-based fusion protein Meso-TR3.
FACS / Human
Mucin 16 is a functional selectin ligand on pancreatic cancer cells.
IP / Human
