ARG22751
anti-Macrophage antibody [BA4D5]
anti-Macrophage antibody [BA4D5] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Pig
Overview
| Product Description | Mouse Monoclonal antibody [BA4D5] recognizes Macrophage This antibody recognizes Porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyeras patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells. It is believed that clone BA4D5 may be specific for Porcine CD68, although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm. |
|---|---|
| Tested Reactivity | Pig |
| Tested Application | FACS, IHC-Fr, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BA4D5 |
| Isotype | IgG2b |
| Target Name | Macrophage |
| Antigen Species | Pig |
| Immunogen | Porcine alveolar macrophages. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | WB: The antibody recognizes a 105 kDa antigen in pig macrophage lysates under non-reducing conditions. FACS: Membrane permeabilization is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
